FastQCFastQC Report
Thu 28 Apr 2016
ERR1042089.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042089.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15741975
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT414980.2636136825271289No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT334730.21263532688877984No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT250390.15905882203471927No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT181460.11527143195183577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA158150.022.6351571
ACGGACC34350.020.2503648
GACGGAC35500.019.0732387
AAGACGG40700.018.2272725
CAAGACG44700.017.506714
CGCAAGA39550.017.4007572
CGGACCA39600.017.2853559
TCTAGCG20700.016.53381728
AGACGGA42800.016.2523376
GTAAACG31350.016.22807127
TCGTTTA24550.015.90020430
GTATCAA225850.015.7682092
TAAACGC32750.015.59083928
GCGCAAG46550.015.3802371
CTAGCGG22350.015.23042529
TTCGGGC34100.014.97360735
CGAACGA16800.014.86607116
GCGAAAG46350.014.76806918
ACGCTTC35200.014.61079531
CGCTTCG36200.014.41160232
Produced by FastQC (version 0.11.5)