FastQCFastQC Report
Thu 28 Apr 2016
ERR1042088.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042088.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413811
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT104632.5284489779150383No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT73341.7723066810693773No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT44401.0729535947570268No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28640.6921033998612893No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA13620.32913576487816903No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA7760.1875252228674443No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7460.18027553641638333No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA7460.18027553641638333No Hit
GTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA7000.16915935052475647No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA5850.14136888579568935No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT5560.1343608555596637No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA5340.12904441882888565No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4820.1164782956470466No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC4760.1150283583568344No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4700.1135784210666222No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA4410.10657039083059656No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA4220.10197892274492462No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA200.001840574937.012
GTGCGCG251.2322607E-437.011
GTATTAC250.005492329629.61
GAGTAGT250.005492329629.61
AAAAGCG250.005492329629.631
CGATTAA250.005492329629.624
GTACGTA250.005492329629.613
CGGACCA1150.027.3478269
TAACGGC556.245482E-726.9090936
AGGCACC556.245482E-726.909098
CGTTCAA358.8597677E-426.4285735
GTATATA358.8597677E-426.428574
ACGGACC1200.026.2083328
GCGCAAG1300.025.6153851
ATTAGGC451.3212136E-424.6666663
GTTTAAC601.3339541E-624.66666633
TTAACGG601.3339541E-624.66666635
CGCAAGA1350.024.6666662
GACGGAC1300.024.1923087
AAGACGG1350.023.2962955
Produced by FastQC (version 0.11.5)