FastQCFastQC Report
Thu 28 Apr 2016
ERR1042087.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042087.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2169071
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT51960.23954955831321334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT47590.21940268437501587No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32460.1496493199162222No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT23760.10953998278525692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC4400.021.4431828
GACGGAC4500.020.9666677
GGTATCA22200.020.3333361
AAGACGG5200.019.567315
AGACGGA5550.018.3333346
CGCAAGA5500.018.1636352
CAAGACG5950.018.0336154
CTAGCGG2800.017.17857229
CGGACCA5850.017.0769219
TTAACGG904.448882E-516.44444535
CGGGTAA1708.54925E-1116.32352824
TCTAGCG2850.016.22807128
CGATTTG5100.015.96078628
TAACGGC1059.350664E-615.85714236
GCGCAAG6700.015.7388051
TAGGACG855.3668773E-415.2352934
TAACCCG1954.1836756E-1115.17948728
GTATCAA30450.014.8850572
CGAAAGC6500.014.80000119
TACGACG4500.014.85
Produced by FastQC (version 0.11.5)