FastQCFastQC Report
Thu 28 Apr 2016
ERR1042086.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042086.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2422818
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT45470.1876740225638079No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT38700.15973135415041495No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT27240.11243106168106726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG1303.274181E-1119.9230771
ACGGACC5400.019.5277798
GACGGAC5550.018.6666667
AAGACGG6000.018.55
CGCAAGA5900.018.52
TACGACG3400.018.4999985
CGGACCA6100.017.5901649
TATACTG3200.016.7656255
TCGTTTA4000.016.6530
ATTGACG4150.016.49397732
TACCGTC3550.016.154937
AGACGGA6550.016.0992376
TCTATAC2900.015.9482763
ACGACGG3850.015.8571446
TAGGTCG1403.4780896E-815.85714221
TAACGCC4550.015.8571424
AACGCAC1059.351568E-615.85714232
GGTATCA27200.015.8474251
TTACTCG2250.015.62222219
AGTACCG1902.7284841E-1115.5789485
Produced by FastQC (version 0.11.5)