FastQCFastQC Report
Thu 28 Apr 2016
ERR1042085.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042085.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4074118
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC52460.12876406623470404No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT52030.1277086230688458No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA49440.12135141888379276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG6050.018.04132328
TATACTG5250.017.2666665
CTAGCGG6350.016.89763629
TTAACGG4600.016.4891335
AAGACGG10750.015.8325595
GGTATCA32500.015.82461551
CGAACGT3750.015.7866664
TAACGGC5000.015.5436
GACGGAC10550.015.431287
GTTCAAA13200.015.4166671
ATTGACG9950.015.24623132
TCGTTTA6200.015.21774230
ACGGACC10700.015.2149528
TAATACT8100.014.8456794
TCGAACG4000.014.83
GTATTAG15750.014.682541
ATTAGAG9050.014.3093923
TACGACG9150.014.1530065
TATTAGA7850.014.1401272
TAAACGC7250.014.03448328
Produced by FastQC (version 0.11.5)