FastQCFastQC Report
Thu 28 Apr 2016
ERR1042084.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042084.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2472422
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT28350.1146648913494541No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG802.7290298E-823.1250025
ACGGACC6350.020.1023628
TAACGCC4200.019.8214284
GACGGAC6400.019.3671887
TACGACG4300.018.55
TCGTTTA4000.018.037530
AATAACG4250.017.847062
ATAACGC4750.017.5263163
GACGGTA4550.017.4835178
TATCTAG4500.017.2666661
ACGACGG4650.017.1075296
CAAGACG7600.017.0394744
CGAACGT2400.016.9583344
AGACGGA7650.016.9281046
AAGACGG7800.016.8397435
GGTATCA16050.016.828661
CGACGGT4750.016.7473687
CGCAAGA7450.016.6375832
CGCATCG4600.016.4891313
GCGCAAG7200.016.4444451
Produced by FastQC (version 0.11.5)