FastQCFastQC Report
Thu 28 Apr 2016
ERR1042083.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042083.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3838424
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT64120.16704772583747912No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT55410.14435612115805863No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT45410.11830376216905689No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA40220.10478258785376499No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC39360.10254208498071084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGAC10250.019.8536597
ACGGACC10350.019.3043488
AAGACGG10650.019.281695
TAAACGC5500.018.16363728
AGACGGA11150.018.0852016
TCGTTTA6650.018.08270630
CGGACCA11850.017.9535879
CAAGACG12450.017.2369464
CGAACGA4250.016.9764716
TTAACGG2950.016.93220335
GTAAACG5800.016.90517227
CGTTTAT7150.016.81818231
ATAACGC9100.016.670333
ATTGACG9750.016.3179532
CGCAAGA12600.016.2976192
ATTAGAG7200.016.18753
GGTATCA44800.016.1462041
TAACGCC7450.016.140944
CGGGTAA5550.016.024
GTATTAG12000.015.8791671
Produced by FastQC (version 0.11.5)