FastQCFastQC Report
Thu 28 Apr 2016
ERR1042082.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042082.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2212036
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC37430.1692106276751373No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT34960.15804444412297086No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA29970.13548604091434316No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG25490.1152332059695231No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA23890.10800005063208737No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC23400.10578489680999766No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG23070.10429305852165155No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC405.9425765E-527.753
GTACCGT705.105443E-621.1428596
GACGGAC6950.019.1654687
AAGACGG7600.018.55
CTAACGC500.00703688818.53
TATACTG3050.018.1967225
TCTAGCG2550.018.13725528
TCTATAC1950.018.0256423
GCGCAAG7750.017.9032271
CAAGACG8100.017.8148164
AGACGGA8100.017.586426
TACGACG4000.017.5755
GTATACG953.609437E-617.5263161
CGCAAGA7750.017.4258082
TGATCGG1301.394983E-817.07692110
TCGTAAG650.00158055917.0769212
CGAACGA4100.016.69512416
CTAGCGG2800.016.51785929
ACGGACC8250.016.3696988
CGGACCA8250.016.3696989
Produced by FastQC (version 0.11.5)