FastQCFastQC Report
Thu 28 Apr 2016
ERR1042081.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042081.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8424544
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT139490.16557572730345999No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT129150.15330206596345156No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT114810.13628037315728897No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC106050.12588218424641143No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA104350.12386427087329593No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG85290.1012399009370715No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT84680.10051582613848299No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80000.018.9393751
AAGACGG25100.018.8685265
GACGGAC24050.018.6923087
TCTAGCG14250.018.56491328
ACGGACC24550.018.5376788
CTAGCGG14200.018.529
CAAGACG26850.017.983244
GTAAACG14800.017.37527
TCGTTTA15350.017.11400630
TAAACGC15250.016.98360628
AGACGGA27500.016.9527266
CGGACCA27500.016.8181829
CGCAATA16200.016.55864136
CGCAAGA28400.016.5457742
TATACTG14100.016.400715
GCGCAAG29450.016.0814951
TATACCG3300.015.696975
GTATTAG22700.015.6475781
CGTTTAT16950.015.6076731
TAACGCC18300.015.4672144
Produced by FastQC (version 0.11.5)