FastQCFastQC Report
Thu 28 Apr 2016
ERR1042080.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042080.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3255405
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT213230.6550029873395169No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT186220.5720332800373533No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT74280.22817437461698314No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74270.22814365647285054No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT42980.13202658348193236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC855.1881216E-821.7647063
TATCTAG8450.018.3905331
ACGGACC8350.018.3892218
AAGACGG8850.017.97745
TACGACG4850.017.9278355
GACGGTA4750.017.9157898
TACCGTC4950.017.5656577
GACGGAC8700.017.0114967
GGTATCA84550.016.935541
CGAACGA4200.016.73809416
CGGACCA9100.016.670339
GCGTAGA1005.8844507E-616.651
ATACCGT6600.016.5378786
TAGAGTC11350.016.4625555
AAAGCCG9700.016.2113415
TATACTG6750.016.170375
AGACGGA9750.015.9384616
ACGGTAT5250.015.8571429
CGTCGTA5500.015.80909210
TAAGACT5750.015.7652184
Produced by FastQC (version 0.11.5)