FastQCFastQC Report
Thu 28 Apr 2016
ERR1042079.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042079.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5141448
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT523011.0172426133649508No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT424920.8264597833139613No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT263710.512909981779452No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153480.2985151264779883No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA81790.15907969894862303No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC78390.15246677589659566No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT77570.15087189445463614No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA60670.11800177693132362No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG60580.11782672896818173No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC60410.11749608281558035No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA59290.1153177081631478No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC55480.10790734439014069No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG55430.1078100955217285No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG53280.10362839418000531No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG15300.021.6437915
ACGGACC14700.021.3945588
GACGGAC15150.020.8811867
CGGACCA16400.019.853669
AGACGGA17400.018.9252876
CGCAAGA16950.018.7728612
CGCGATA701.22024285E-418.514
CAAGACG19300.018.116584
GCGCAAG18300.017.9945351
TGCGACG2100.017.61904722
GCAAGAC23400.017.1559833
TAGACAG6500.016.5076925
GCGAAAG19300.016.1994818
CAGAGCG20750.015.95903614
TCGTTTA11750.015.90212930
TACCGTC12850.015.8365767
TTCGGGC9350.015.828876535
GAGCGAA20150.015.79156416
TAAACGC9400.015.74468128
CGAACGA10550.015.60663516
Produced by FastQC (version 0.11.5)