FastQCFastQC Report
Thu 28 Apr 2016
ERR1042078.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042078.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5228554
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT999281.9111976274893594No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT777261.4865677967560438No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT455440.8710630128329936No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310270.5934145463544988No Hit
GTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA135030.2582549592105198No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA126590.24211282890068653No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT75540.14447589142237033No Hit
GAGTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAA65950.1261343002290882No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT62170.1189047679339259No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAA59790.11435284019252741No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA53810.10291564359859343No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC53660.10262875739640444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGAC11900.020.0546237
ACGGACC12100.019.723148
AAGACGG13550.018.977865
GCGCAAG14150.018.1731451
CGCAAGA13850.018.1660672
CGGACCA14200.017.3274659
TCGTTTA8350.017.28143730
TATACTG8350.017.2814375
AGACGGA14500.016.841386
CCGGTCG8750.016.70285820
CGCATCG8700.016.16092113
TAACGCC10250.015.8829284
CAAGACG16300.015.7760734
ATCGCCG9350.015.43315416
GCGGGTA7200.014.90277823
TCGCCGG9700.014.87628817
AATAACG10150.014.7635472
ACACGCT16700.014.7335339
ATGGTCG8550.014.7134536
GAACAGT41900.014.5704051
Produced by FastQC (version 0.11.5)