FastQCFastQC Report
Thu 28 Apr 2016
ERR1042077.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042077.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7539341
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT269890.35797558433820675No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT234010.3103852180183918No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT154520.20495159988120978No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77700.1030594053246829No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT77020.1021574697311078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC16950.018.5545718
GACGGAC17150.018.3381927
AAGACGG17950.018.1392755
TAACGGC5650.016.69911636
CGGACCA19600.016.5178599
GGTATCA121350.016.5105061
TTAACGG5950.016.47899235
AGACGGA19250.016.4337656
CGCAAGA20500.016.4243892
CTAACGC1504.6838977E-916.0333333
CAAGACG22600.015.7986734
TAACGCC14050.015.6690394
GCGCAAG23300.015.6416321
TCGTTTA12650.015.35573230
TCTAGCG8650.015.18497128
TACCGTC12200.015.0122947
CTAGCGG8850.014.84180729
GTATTAG24900.014.7851411
TATACTG13850.014.6931415
CGCATCG13300.014.60526313
Produced by FastQC (version 0.11.5)