FastQCFastQC Report
Thu 28 Apr 2016
ERR1042076.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042076.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5257660
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT337110.6411787753487292No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT296380.5637108523563715No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT188950.3593804087750064No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101420.19289950282064644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC9600.018.1145828
GACGGAC10250.016.9658537
GGTATCA128700.016.8900551
AAGACGG11000.016.481825
CGGACCA11100.016.3333349
AATCGTC3550.016.1549328
ATTAGAG11250.016.1155553
TTAACGG5800.015.31034635
GCGCAAG12350.015.1295541
CGCAAGA11650.015.0858362
GTATTAG17900.014.98603251
TATACTG8550.014.9298255
TCTAGCG4500.014.828
TAACGGC6250.014.50436
AGACGGA11900.014.4579836
ATTGACG9350.014.44385132
TTTAGCG2850.014.28070226
CGTATTA3900.014.23076815
GTACTAG4000.013.8750011
TAGGTCG2551.8189894E-1213.78431321
Produced by FastQC (version 0.11.5)