FastQCFastQC Report
Thu 28 Apr 2016
ERR1042075.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042075.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2533464
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT310641.2261472829296172No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT243970.96298980368381No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT152410.601587391808212No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73800.29130076448688436No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA32130.12682240600221673No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA29660.11707290887101612No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC28700.11328363063378835No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG400.001931825323.1250025
ACGGACC3200.020.81258
TTTAGCG1550.020.29032126
CGACGAG1101.7571438E-920.18181824
CAATGCG902.1542983E-618.519
CGACTAT1205.182301E-918.536
TATACTG2850.018.1754385
TAACCGG953.6098572E-617.52631622
CTTACCG953.6098572E-617.5263169
CGGACCA3800.017.5263169
GTCGACG852.7252127E-517.41176436
CGTTAGA752.068505E-417.2666662
AATCGTC1301.3953468E-817.07692328
AATACTG5200.016.7211535
TGCGACG1005.883412E-616.65000222
GACGGAC4150.016.4939777
GACCGTG4300.016.3488397
CGCAAGA4200.016.2976192
ATTGACG3900.016.12820632
CGATAGA2300.016.08695637
Produced by FastQC (version 0.11.5)