FastQCFastQC Report
Thu 28 Apr 2016
ERR1042074.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042074.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2625102
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT462431.7615696456747205No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT369401.4071834161110692No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT211390.8052639478389791No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103600.3946513316434942No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA40760.15527015712151376No Hit
TATCAACGCAGAGTACATGGGGGGTTGTGATCACTTCGCCCTC40110.15279406285927175No Hit
GGTATCAACGCAGAGTACATGGGGGGTTGTGATCACTTCGCCC39420.15016559356550715No Hit
CCACAACACAGCTGAGGGCGAAGTGATCACAACCCCCCATGTA37320.14216590441057148No Hit
GTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA33870.12902355794174855No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30890.11767161809331599No Hit
GTATCAACGCAGAGTACATGGGGGGTTGTGATCACTTCGCCCT28440.10833864741255769No Hit
GCTGAGGGCGAAGTGATCACAACCCCCCATGTACTCTGCGTTG28280.10772914728646733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT1301.8189894E-1221.34615330
CGCCACA9700.020.5979391
ATACCGC2200.019.34090827
GTATGCG1409.458745E-1118.533
TCTACCG500.00703723118.49999837
GTCCGAT500.00703723118.49999830
ATCGGTC500.00703723118.49999826
TATACTG3150.018.2063485
TCGGCCG1750.017.97142827
ACGTGCG1451.546141E-1017.8620689
GCGGGTA4100.017.14634123
TCCGATC650.001580664517.07692331
ATACTGG3250.017.0769236
CTAACGG1301.3953468E-817.07692327
TGCGTCT2950.016.93220337
GGCGTCG2200.016.8181828
CGGGTAA4200.016.73809624
TAACGGA1352.222805E-816.44444528
CGCGGGT4300.016.34883722
TTAACGG2300.016.08695635
Produced by FastQC (version 0.11.5)