FastQCFastQC Report
Thu 28 Apr 2016
ERR1042073.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042073.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3995698
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT367040.9185879413309015No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT298520.7471035098248165No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT183330.4588184592529266No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87920.22003664941644738No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA47800.11962866062450166No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC42570.10653958332186267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACG1800.021.58333222
CAATGCG2000.020.3519
CGTTCGG1457.2759576E-1219.1379324
TGCGTAC609.2422764E-418.516
GTACTAG3150.018.206351
TAGGTCG2250.018.08888821
CGACGAG2050.018.04878224
ACGGACC2900.017.8620688
TATACTG5300.017.8018885
TAACCGG2200.017.6590922
CGCGATA1401.873559E-917.17857214
TCTATAC6200.017.0080643
TTGCGAT1201.0430813E-716.95833216
CTAGTAC3500.016.9142863
CGGTAAG1655.4569682E-1116.81818228
ATGTACG3100.016.70967911
GTCGTCA2550.016.68627424
CGACTAT1801.0913936E-1116.44444536
GCCGAGT3050.016.3770512
CGCTACG803.3851888E-416.187516
Produced by FastQC (version 0.11.5)