FastQCFastQC Report
Thu 28 Apr 2016
ERR1042072.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042072.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2366678
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT42290.1786892851499021No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35470.14987252173721985No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT26050.11006989543993734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15450.024.9061471
TATAGCG450.003826987720.5555557
TTTAGCG1101.7571438E-920.18181826
ACGGACC3100.017.9032258
TATATCG2050.017.1463435
ACCCGTT2500.017.0230
ATTGACG2750.016.81818232
GTATCAA23300.016.673822
GACGGAC3450.016.6231887
CGGGTAA2050.016.24390424
TCTAGCG1403.4779077E-815.85714328
TATACCG1201.9375111E-615.4166665
ATAACGC2500.014.7999993
GTCCGTA2151.2732926E-1114.62790723
TTAACGG1406.002083E-714.53571535
CGGACCA3700.014.59
CTAGACT4150.014.265064
AGACGGA4100.013.9878056
AACCCGT3050.013.9508229
CGCGCTA3050.013.9508224
Produced by FastQC (version 0.11.5)