FastQCFastQC Report
Thu 28 Apr 2016
ERR1042071.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042071.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1435675
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT81430.5671896494680203No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT79700.5551395684956554No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT38470.2679575809288314No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23620.1645219147787626No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC20050.13965556271440263No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA15500.10796315322061051No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT14650.1020425932052867No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG603.7271195E-521.58333210
GGTATCA30900.020.5954691
GGCGTAA555.1439507E-420.18181813
CCCGTAT701.2194655E-418.58
ATTGACG2900.018.532
TAGACCG500.007035715518.49999825
TTGACGG3150.017.03174633
TAGACTA1851.8189894E-1217.05
CGCCGTA904.4471955E-516.44444525
CGCTAGA2500.016.27999937
GACGGAC2850.016.228077
GTATCAA39900.015.9498752
TCGCTAA700.00259291515.85714314
AAGCGAC957.0616356E-515.57894831
GTTCGGC957.0616356E-515.57894827
TGACGGA3550.015.11267634
AGTATGG4800.015.0312492
TATAGAC1252.9598032E-614.7999993
GTATACT1651.6456397E-814.5757574
GTTAGCC1152.2104909E-514.4782613
Produced by FastQC (version 0.11.5)