FastQCFastQC Report
Thu 28 Apr 2016
ERR1042070.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042070.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1340763
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT108930.8124478375372829No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT100570.7500952815672867No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39900.29759174440225455No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31600.23568669481481816No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT16520.12321342399812645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36100.022.189751
CGCAAGA4100.022.1097562
ACGGACC3900.021.820518
CGCACGT450.003826134420.55555730
TATACTG2200.020.1818185
GACGGAC4200.019.8214287
CGGACCA4300.019.3604649
AAGACGG4900.019.2551045
AGACGGA4700.018.8936166
GCGCAAG4750.018.3052641
ATACTGT2800.017.8392856
GTATCAA45250.017.6209952
CGATACG852.723664E-517.41176426
CGAAAGC4850.017.1649519
TATACCG650.001580119117.0769235
AACACCG650.001580119117.0769235
CAAGACG5650.016.6991164
GCGAAAG5050.016.4851518
ATTGACG2150.016.34883732
GGCGTTA1059.345342E-615.85714231
Produced by FastQC (version 0.11.5)