FastQCFastQC Report
Thu 28 Apr 2016
ERR1042069.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042069.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2863262
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT137220.47924360397337024No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT125400.4379620167487292No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT93070.3250488428931757No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47050.1643230692825176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38200.025.8612581
TTAACGG701.2200034E-418.535
CGCAACG650.001580712317.07692312
CTAGCGG1851.8189894E-1217.00000229
ACGGACC4900.016.9897968
GTATCAA58550.016.9359512
GACCGTA904.4496825E-516.44444535
GACGGAC5350.015.9065437
TAACGGC1059.352752E-615.85714436
CGCGTAC700.0025937615.85714210
CGGGTAA2650.015.35849124
TATACTG6250.015.0965
TATACCG1353.977857E-715.0740755
TAACCCG2850.014.92982428
CGTTATT2900.014.6724142
TCTAGCG1904.5656634E-1014.60526428
ACACGCT7050.014.4326259
GCGGGTA2950.014.42372923
CGAACGA2450.014.34693816
CGGACCA6100.014.2540999
Produced by FastQC (version 0.11.5)