FastQCFastQC Report
Thu 28 Apr 2016
ERR1042068.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042068.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1645306
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT332752.0224201455534714No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT293511.7839234768486836No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106030.6444393930369183No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT86490.525677290425003No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT28970.17607666902083868No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT24140.14672042768943894No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19980.12143637718454803No Hit
GAGTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAA18270.11104317373181645No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAA16770.10192632859784137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAA1054.0017767E-1122.9047624
GACGGAC4500.021.7888897
ACGGACC4650.021.0860218
AAGACGG5450.019.3486235
CGCAAGA5400.019.1851842
CAAGACG6050.018.9586774
CGTACGC609.239126E-418.53
GAACCGA609.239126E-418.56
TACACCG902.1533651E-618.55
GCGCAAG5450.018.3302751
CGGACCA5450.018.3302759
ATTGACG2950.017.55932232
TCGTTTA1900.017.52631630
AGACGGA5700.017.5263166
GGTATCA112500.017.3653321
TTGACGG3000.017.26666633
GCGGGTA1401.87174E-917.17857223
TACGACG1301.3942554E-817.0769235
GCAAGAC7750.016.7096773
TACGCTG1059.347923E-615.8571425
Produced by FastQC (version 0.11.5)