FastQCFastQC Report
Thu 28 Apr 2016
ERR1042067.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042067.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4436208
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT213000.48013979506822047No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT206360.4651720568557651No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT150780.3398848746497008No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72210.16277415306045162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68200.023.2470681
ACGGACC8200.018.274398
GACGGAC8600.017.6395367
TATACTG8300.017.3855425
TACCGTC5200.016.7211537
AAGACGG9050.016.558015
CGGACCA9100.016.2637359
GTATACG1951.8189894E-1216.1282061
GTATCAA98850.015.96408752
AGACGGA10200.015.5980396
CGCAAGA9400.015.3510632
CGTCGTA5000.015.1710
TCTATAC6350.015.1496083
TCTAGCG3800.015.09210628
CGAAAGC9400.014.95744619
ACCGTCG6100.014.8606568
GTAAGAC4650.014.7204313
CTAGCGG3900.014.70512929
GCGAAAG9700.014.49484518
TATACCG1702.4421752E-814.1470595
Produced by FastQC (version 0.11.5)