FastQCFastQC Report
Thu 28 Apr 2016
ERR1042066.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042066.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1675164
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT127750.7626118994916319No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT124720.7445241182355877No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51470.30725349876191227No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39480.23567841715796184No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT29840.17813181276579487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44300.022.0496621
TCTATCG806.9640373E-720.812529
GACGGAC6200.020.588717
AAGACGG6700.020.1567175
ACGGACC6400.019.9453128
CGGACCA6550.019.7709929
GCGCAAG7050.018.6312071
GTATCAA52450.018.6234512
CGCAAGA6900.018.52
AGACGGA7050.018.1063826
ATGGTCG2250.018.08888836
GCGAAAG7400.017.518
ACCGATC650.001580341617.0769238
GTAGCAC1201.0420081E-716.9583343
CCGATAA2300.016.8913049
CGAAAGC7400.016.7519
CCGAGTT1005.881022E-616.6513
AGCGAAA7600.016.55263117
CGTAGAC1352.221168E-816.4444453
CAAGACG8150.016.3435574
Produced by FastQC (version 0.11.5)