FastQCFastQC Report
Thu 28 Apr 2016
ERR1042065.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042065.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2017962
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT232071.150021655511848No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT189450.9388184713091724No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT111140.5507536811892395No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92530.45853192478351923No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20440.10129031171052774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG909.487303E-820.55555535
AAGACGG3000.018.55
ATATCGC1205.180482E-918.56
TAACGGC1002.8776049E-718.49999836
CGATGCG1950.018.02564233
ACGGACC2750.017.4909088
TATATCG1502.5102054E-1017.2666665
GTACTAG1401.87174E-917.1785721
GACGGAC2800.017.1785727
TATACGG904.448637E-516.4444452
TACCGTC2150.016.3488377
TGCGACG803.3841506E-416.187522
GTATACG803.3841506E-416.18751
ACACGCT4000.016.1874989
CGCAAGA3000.016.0333332
TCGTTTA1851.8189894E-1116.030
AGTACCG1302.5916052E-715.6538465
CGGCGAT2250.015.62222230
CGGACCA3250.015.369239
TATACTG3300.015.1363645
Produced by FastQC (version 0.11.5)