FastQCFastQC Report
Thu 28 Apr 2016
ERR1042064.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042064.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5716391
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT115410.20189311752817468No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT100210.17530291402390075No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT72180.12626847953542716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39950.023.3391741
ACGGACC7700.017.538968
GTATCAA58200.015.98883152
GACGGAC8350.015.9520967
GGCGTTA2900.014.67241431
AAGACGG11350.014.6696035
TATACTG9900.014.2020215
ATAACGC5850.013.5982913
TACGACG4900.013.5918385
TACGTAG3300.013.45454414
CGCAAGA10800.013.3611122
TAGAACG1951.0284566E-813.2820524
CGGACCA10450.013.2775129
CGCGCTA6600.013.17424124
TACGTCG2254.0745363E-1013.15555621
TAGACAG9850.012.9593915
CTAGCGG4450.012.8876429
TAACGCC4750.012.8526324
TCTAGCG4500.012.74444528
TCGTTTA3950.012.6455730
Produced by FastQC (version 0.11.5)