FastQCFastQC Report
Thu 28 Apr 2016
ERR1042063.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042063.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9283793
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT203190.21886528491102722No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT182660.1967514786251697No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT134650.1450377017238536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72450.021.9855061
ACGGACC13150.015.8973398
GACGGAC13950.014.9856627
GTATCAA108150.014.6597332
TATACTG16350.014.596335
TAACGCC10100.014.1039614
TACGACG11050.014.0633495
TCTAGCG7150.013.97202828
TACCGTC10050.013.8059717
CGAACGA6500.013.66153916
CTAACGC1951.0288204E-813.282053
CGGACCA15900.013.2641529
CGTCGTA9500.013.242105510
TCTATAC14300.013.1958053
GTACTAG5050.013.1881191
GTAAACG8700.013.18390827
AAGACGG18700.013.0588235
GTATAGG14350.012.7630671
ATAACGC12250.012.6857153
GTCTTAC9350.012.6631011
Produced by FastQC (version 0.11.5)