FastQCFastQC Report
Thu 28 Apr 2016
ERR1042062.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042062.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9718303
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT185990.19138114956901425No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT168310.17318867296070106No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT126050.1297037147329117No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66500.024.3421061
GTATCAA103200.015.649712
ACGGACC16100.015.627338
GACGGAC15950.015.3103447
TAACGCC11650.015.2446364
TCGTTTA11300.015.06194630
TCTAGCG7550.014.21192128
TCTATAC14400.014.0034723
TATACTG17700.013.796615
CGAACGA7650.013.54248316
AAGACGG19400.013.5412375
CTAGCGG8100.013.47530829
AATAACG13150.013.3650192
AACGCCG13350.013.303375
ATAACGC15350.013.2573293
CGGACCA18950.013.17941959
ATACCGA4250.013.0588246
CGCATCG13350.013.026216513
TATCCCG5850.012.9658125
CGCAAGA19700.012.8654812
Produced by FastQC (version 0.11.5)