FastQCFastQC Report
Thu 28 Apr 2016
ERR1042061.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042061.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14882661
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT561420.37723092664678715No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT532970.35811472155416296No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT382110.2567484403494778No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215500.14479937425168793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA146700.026.2177931
GTATCAA225550.017.0933272
ACGGACC15450.015.2071198
TATACTG23050.014.6073745
GACGGAC17350.013.7550447
AATCGTC12400.013.72580728
TCTATAC25300.013.1620563
TCGCTAA5600.012.88392914
CTAATCG13450.012.791821526
GTATTAG38900.012.555271
ATCAACG311300.012.4620934
TCAACGC316050.012.2806525
TATCAAC323600.012.1027513
TAGACAG26550.012.0546145
CAACGCA321700.012.0362146
GTATACG7150.011.9020981
CTAGTAC8750.011.843
TATACCG6250.011.845
CGAACGA9600.011.75520816
CGAACGT4300.011.6162794
Produced by FastQC (version 0.11.5)