FastQCFastQC Report
Thu 28 Apr 2016
ERR1042060.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042060.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8943687
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT130720.14615896106382076No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT125980.1408591333753071No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52450.024.302191
TCTAGCG7500.016.27999928
GACGGAC12900.015.7751947
CTAGCGG7750.015.75483829
GTATCAA81200.015.6748772
TATACTG16100.015.5124235
TCTATAC13350.015.2434463
CTAGTAC5650.014.7345133
ACGGACC13900.014.6402888
GTATTAG21750.014.459771
CGAACGA6400.013.87500116
CTAACGC1601.7875755E-713.8750013
TAACGCC9200.013.8754
CGCAATA9300.013.52688136
CGCAAGA16200.013.3611112
TCGTTTA8600.013.33720930
GTACTAG5600.013.2142861
AGACGGA16850.013.0652816
TAGACCG2305.7661964E-1012.8695655
ATACCGC12700.012.81889827
Produced by FastQC (version 0.11.5)