FastQCFastQC Report
Thu 28 Apr 2016
ERR1042059.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042059.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5690532
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT108060.18989437191461184No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT98910.17381503170529575No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT66740.11728253175625758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36600.025.3743171
GTATCAA55850.016.6615922
ACGCGCG9100.016.26373521
CGCGCTA9350.015.63101624
GACGGAC9450.015.4656097
TATACTG12000.015.4166675
TCTATAC6750.015.3481483
GTACTAG3450.015.0144931
TCGAACG3150.014.682543
TACCGTC6600.014.5757597
ACGGACC10250.014.4390248
GTATTAG13450.014.0297391
CGAACGT3400.013.60294154
CCGTCGT7100.013.5492959
TCGTTTA6600.013.45454630
AAGACGG12250.013.4408165
CGTCGTA6750.013.4296310
GCGCGCT10950.013.34703223
TACGACG7050.013.1205675
ATACTGT16600.013.0391576
Produced by FastQC (version 0.11.5)