FastQCFastQC Report
Thu 28 Apr 2016
ERR1042058.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042058.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8765072
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT96930.1105866557627821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43600.023.6766051
ACGGACC19550.016.6547328
GACGGAC19000.016.4552637
AAGACGG22550.016.0798235
CGAACGA11400.015.90350816
TCGTTTA13550.015.70110630
GTATCAA66700.015.3935532
ACGAACG11800.015.05084915
TAACGAA12150.014.76954713
AGACGGA22100.014.6493226
ATAACGA12250.014.49795912
CGCAAGA22900.014.4606992
TACCGTC14100.014.4326247
TCGCTAA2700.014.38888814
CGGACCA22750.014.3120899
TATACCG3500.014.2714295
CGTTATT11800.014.2669512
TACGACG12750.014.2196075
GTATTAG22000.014.2113631
TAACGCC14950.014.1070234
Produced by FastQC (version 0.11.5)