FastQCFastQC Report
Thu 28 Apr 2016
ERR1042057.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042057.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476569
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT95662.0072644255081635No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT91791.9260589757202No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT67291.4119676269333505No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31850.6683187534229041No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10010.2100430367900556No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA9760.20479720670039386No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9250.19409571331748393No Hit
GTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA9230.19367604691031098No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7050.14793240852846073No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA6860.14394557766031782No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6070.12736875457698676No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6050.12694908816981382No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5630.1181360936191821No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATA200.001840855237.026
CGCATAG250.005493154329.627
CTCGCTA250.005493154329.613
ATAGTCG250.005493154329.630
CGCCGAA250.005493154329.633
TATCGCG250.005493154329.616
GTTTACC400.001929537723.12515
CGGACCA400.001929537723.1259
CGCCGAG502.6988864E-422.220
GTACTAG450.003822571820.5555551
CGCCAGC450.003822571820.55555534
ATATAGC555.137025E-420.1818186
GCTTAGA851.2421806E-619.5882361
CATTGCG609.226003E-418.529
ATTACGG500.007028986218.58
GTTAACT500.007028986218.54
CATAGCT500.007028986218.52
GAACACC500.007028986218.533
ACCCAAT500.007028986218.521
AGAGCGT609.226003E-418.55
Produced by FastQC (version 0.11.5)