FastQCFastQC Report
Thu 28 Apr 2016
ERR1042056.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042056.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences696303
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT76311.0959309381117128No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT74611.071516279550713No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT54790.7868700838571713No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22720.32629473088583566No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7380.10598834128245893No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACC250.005494873529.61
TGATCGC358.865869E-426.4285729
GTGAGCG451.3224804E-424.6666669
GTCGGTA451.3224804E-424.66666626
GCCGAGT551.901262E-523.54545412
ATACCGT952.8012437E-1023.3684226
CTAACTG502.7006198E-422.24
TAACGCC705.0971576E-621.1428594
AATACTG1750.021.1428575
CTAACGC450.003824316220.5555553
TCTAACG450.003824316220.5555552
CGATATA450.003824316220.55555514
CTGTGCG450.003824316220.5555559
GGTAAAC1101.7516868E-920.18181835
CCGATAC555.1402947E-420.1818183
CCGCCCT555.1402947E-420.1818189
TACCGTC555.1402947E-420.1818187
CAATACC656.897137E-519.9230774
CTCTATA951.6737431E-719.4736842
GCTCGGA1052.2555469E-819.38095311
Produced by FastQC (version 0.11.5)