FastQCFastQC Report
Thu 28 Apr 2016
ERR1042055.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042055.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences633313
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT36180.5712814990376007No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT34030.5373330406923591No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15280.24127090396060086No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13070.2063750467778176No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGAGC250.00549450429.59999830
TACGCTA358.8649814E-426.428579
ACGCTAT358.8649814E-426.4285710
AAGACGG1450.025.5172425
ATTAGAC451.3222957E-424.6666663
TCGCGGG551.9009098E-523.54545433
CTTCGCG603.7228856E-521.58333431
AATAACG603.7228856E-521.5833342
GGTATCA13250.020.664151
ATATACG450.003823939520.5555556
TATACGC450.003823939520.5555557
TTCGCGG656.8958776E-519.92307732
TAACGCC609.230573E-418.54
CGCAAGA1601.8189894E-1218.52
ATAATCG500.007031480318.49999815
ATACGCT500.007031480318.4999988
CAAGACG1950.018.0256424
TTGACGG1258.556526E-917.7633
AGTACCG953.6012134E-617.5263165
ATAACGC852.7199909E-517.4117643
Produced by FastQC (version 0.11.5)