FastQCFastQC Report
Thu 28 Apr 2016
ERR1042054.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042054.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1290535
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT75330.5837114065097033No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT70360.5452002464094348No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT52840.4094425955127138No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25080.19433800710558025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26500.019.7566051
GTACCGT500.007035337418.56
TATCGGT500.007035337418.524
CCGTATT953.6070815E-617.52631614
CTCGCTA650.00158007617.07692313
TTACGCC650.00158007617.07692334
ATCGGTT650.00158007617.07692325
TTACCGA650.00158007617.07692310
TAACGGC1005.8789137E-616.65000236
CGGGTAA904.4466364E-516.44444524
CGCCGAT1151.242408E-616.08695637
AGGCCGT700.002592724515.8571436
TAGCGAG1059.344796E-615.85714228
GGACGGA1302.589477E-715.6538466
TAATCGG957.060749E-515.57894816
AAGACGG2150.015.4883725
TTAATCG1201.9358504E-615.41666637
TACCGTG1201.9358504E-615.4166667
CGCAAGA2055.456968E-1215.3414632
GTACTAA855.3647865E-415.2352931
Produced by FastQC (version 0.11.5)