FastQCFastQC Report
Thu 28 Apr 2016
ERR1042053.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042053.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5711310
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT323970.5672428917358714No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT257990.45171773200894366No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT257560.450964839940399No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108360.18972880127326305No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA68700.1202876397884198No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC62770.10990473288965227No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT58900.10312870427275005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114950.019.7633741
CGTTCGG1457.2759576E-1219.1379324
CTAGCGG10650.018.06572729
TCTAGCG10900.017.65137728
TGCGACG2000.016.65000222
AAGACGG13400.016.5671635
CGAACGA7700.016.33766216
CGCAATA12700.016.1692936
ACGGACC12900.016.0620168
GACGGAC12850.015.83657557
CGGACCA13450.015.542759
CGAGCCG14550.015.3848815
ACGAACG8450.015.10650915
CGTTATT11300.015.0619472
TTAACGG3700.015.035
CGGTCCA13550.014.74538810
CGCAAGA14650.014.6484642
TAGCGGC13950.014.18996430
GTATTAG18950.014.1556731
TAACGAA9100.014.027472513
Produced by FastQC (version 0.11.5)