FastQCFastQC Report
Thu 28 Apr 2016
ERR1042052.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042052.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5328266
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT334010.6268643494900593No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT260340.4886017327213018No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT250550.4702280254026357No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96690.18146616554053419No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96800.022.5516551
TGCGACG2500.019.2422
ACGGACC6950.015.9712228
TAGGTCG3150.015.85714321
CGGACCA7600.015.3355269
TCTAGCG6300.015.26984228
GACGGAC7650.014.99346357
GTATCAA146500.014.9515352
CGGTCCA6900.014.74637710
CTAGCGG6400.014.74218829
AAGACGG9450.014.4867735
CGAACGA4600.014.07608716
CGCAATA6800.013.87536
CGACGAG4150.013.81927724
CGACTAT1753.5741323E-813.74285836
TATATCG3800.013.6315795
TACCGTC6150.013.5365867
TCTAACG2051.4279067E-913.5365852
AATGCGA4250.013.49411820
CGATTTG8950.013.43575528
Produced by FastQC (version 0.11.5)