FastQCFastQC Report
Thu 28 Apr 2016
ERR1042051.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042051.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4044809
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT491801.2158794148252736No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT401640.9929764297893918No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT337110.8334386123053029No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144180.3564568809058722No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51760.12796648741634029No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA146750.022.0487231
ACGGACC5750.018.9826098
GACGGAC5850.017.3931647
CGGACCA6100.016.9836069
AAGACGG6500.016.2230765
CGGTCTA2450.015.85714220
GTATCAA213700.015.1324292
GCGCAAG6900.014.7463761
CGTTATT5300.014.66037752
AGACGGA7350.014.59863956
GTACCGT1152.212456E-514.4782616
GCGTAGA1152.212456E-514.4782611
TCGTTTA5150.014.36893230
TCTAGCG2750.014.12727228
TATACTG6650.013.6315785
GCGTTAT5300.013.6132081
TACGACG4250.013.4941185
GTATTAG7700.013.4545451
ACGACCG2202.8558134E-1013.45454530
CGCATCG5650.013.42477813
Produced by FastQC (version 0.11.5)