FastQCFastQC Report
Thu 28 Apr 2016
ERR1042050.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042050.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8453643
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT302590.3579403577842121No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT238040.28158274485922813No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT236490.2797492158114555No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103410.12232596053559394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG11750.016.05957428
CTAGCGG11750.016.05957429
ACGGACC13500.016.0333338
GGTATCA134400.015.93973351
AAGACGG15500.015.3967735
GACGGAC13950.015.38351257
CGCAATA13650.014.637362536
CGGACCA15200.014.6052649
CTAGTAC5650.014.0796453
GCGTTAT12650.014.0395261
TAACGCC12000.013.5666664
TCTATAC11500.013.5130433
TCGTTTA11300.013.42477830
CGCAAGA17250.013.2985512
CAAGACG20300.013.2142864
GTATTAG23400.013.2029911
AGACGGA17750.013.1323946
GTAAACG10000.012.76499927
CGGTCCA16500.012.55757510
GCGCAAG18650.012.498661
Produced by FastQC (version 0.11.5)