FastQCFastQC Report
Thu 28 Apr 2016
ERR1042049.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042049.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2357104
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT55530.23558570177641713No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT50960.21619750337702534No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT43760.18565154528607988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17150.024.5947511
CGAACGA2050.017.14634316
GTATCAA24950.016.979962
TACCGTC2700.016.4444457
ACGTTCG803.3844527E-416.18757
TAACGAA2200.015.97727313
ACGAACG2250.015.62222215
GACGGAC3250.015.3692317
AGATCGT855.367122E-415.2352946
ATACCGT3650.015.2054816
ACGGACC3300.015.1363648
TCCGATA2450.015.102048
AACGAAC2350.014.95744614
TTAACGG750.004106108614.79999935
CCGATAA2400.014.6458339
AATACCG1152.2116814E-514.4782615
CGGACCA3450.014.4782619
GTACGTA1304.448446E-614.23076913
TACGACG2650.013.9622645
AAGACGG3900.013.7564115
Produced by FastQC (version 0.11.5)