FastQCFastQC Report
Thu 28 Apr 2016
ERR1042048.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042048.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2598663
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT74270.2858008137261353No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT67820.2609803579763902No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT57410.2209212968361038No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26870.10339932496056625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23850.024.4339641
AATCGTC1401.873559E-917.17857228
GCGTTCG650.001580658817.07692323
GTATCAA36200.015.8425422
TTAACGG1201.9376894E-615.41666635
ACGGACC3950.014.9873428
TAACGGC1252.962037E-614.80000136
TATACGA1001.0942064E-414.85
TCTATAC5500.014.83
CTAATCG1651.6478225E-814.57575726
GCGCAAG4700.014.5638281
TAGGACG1406.002738E-714.5357154
TATATCG1152.211854E-514.4782615
GACGGAC4100.014.4390247
TATACTG6550.014.404585
TACCGTC2850.014.2807027
CGGACCA4500.013.97777759
AAGACGG4900.013.9693895
GCGTAGA800.006301440313.8751
GCATTCG2551.8189894E-1213.78431322
Produced by FastQC (version 0.11.5)