FastQCFastQC Report
Thu 28 Apr 2016
ERR1042047.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042047.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1714987
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT53640.31277205016714416No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT49260.28723249797228784No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40220.23452072814546115No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19310.112595605680976No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACGC450.00382656420.5555573
GCGTAGA656.904661E-519.9230771
GGTATCA19500.019.2589741
TTTAGCG902.1534725E-618.526
AAGACGG4250.017.847065
TAATACG953.608482E-617.5263164
GACGGAC3850.016.8181827
GTATACG904.4480086E-516.4444451
ATAACGC3300.016.2575763
ACGGACC4150.016.0481938
TAACGCC2550.015.9607844
CGCAAGA4300.015.9186062
GTAAACG1751.3278623E-1015.85714327
TATAACG700.002593191615.8571422
GCGAGTG700.002593191615.8571429
GTATTAG5100.015.5980391
TCGTTTA2500.015.5430
TAGCGGG1201.9367544E-615.41666628
CGGACCA4450.015.3820239
CTAGCGG1701.4879333E-915.23529429
Produced by FastQC (version 0.11.5)