FastQCFastQC Report
Thu 28 Apr 2016
ERR1042046.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042046.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855381
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24000.28057672545918133No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21640.25298668078902853No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18800.21978510160969206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6000.026.2083341
AATGCGA451.3228254E-424.66666820
TGCGACG502.7013203E-422.19999922
TATATCG450.003825019320.5555575
TAGACAG1101.7535058E-920.1818185
ACAGCGT555.141614E-420.1818188
CGACCAT609.234181E-418.510
GTCTAAG801.6158829E-518.51
ATGCGAC701.2187346E-418.521
TATACTG1601.8189894E-1218.55
GTGCTCG500.00703344918.4999981
GTATTAG1950.017.0769231
ATAGACT904.4438166E-516.4444454
TTATACT2250.016.4444454
TAGACTG1151.2412092E-616.0869565
GCGTTAT700.002591766615.8571421
TCTAATC700.002591766615.8571423
TAAGACT1059.337429E-615.8571424
GTACGCC700.002591766615.85714236
GTTCTAA1302.5864938E-715.6538461
Produced by FastQC (version 0.11.5)