FastQCFastQC Report
Thu 28 Apr 2016
ERR1042045.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042045.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2378277
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT48740.2049382809487709No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT46040.19358552431024645No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT38600.1623023726840902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTA1457.2759576E-1219.13793215
GTCGTAC500.007037041218.4999981
GACGGAC5900.018.186447
GGTATCA19800.018.032831
AAGACGG6200.017.9032255
ACGGACC6400.017.6328128
CGAACGT1800.017.4722234
GTAAACG3050.016.37704827
CGGACCA6950.016.237419
AGACGGA7000.016.121436
ATACCGT4850.016.0206186
ATAACGC4050.015.9876553
GCGCAAG7200.015.6736121
CGCAAGA7100.015.6338032
TACCGTC3800.015.5789487
CGTCGTA3700.015.50000110
CGCACTA1201.937522E-615.41666629
CTTACAC5300.015.3584913
TAACGCC3550.015.1126774
TAAACGC3350.014.91044828
Produced by FastQC (version 0.11.5)