FastQCFastQC Report
Thu 28 Apr 2016
ERR1042044.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042044.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2226393
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT49320.22152423224471152No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT43600.19583245186272144No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36140.16232533968620994No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTTA701.21984885E-418.513
GGTATCA20400.016.5955891
CGAACGA1901.8189894E-1216.55263116
TATACTG4400.016.3977285
GACGGAC3950.016.3924057
ACGGACC3900.016.1282068
CTAGCCT3100.016.1129044
TTAACGG1151.2433975E-616.08695635
AAGACGG4500.016.0333335
ACGAACG2055.456968E-1215.34146315
TATACCG855.3669565E-415.2352935
TCTAGCC2800.015.1964283
CCGTCGT2800.015.1964289
TCTATAC3900.015.1794873
TAACGGC1353.976711E-715.07407336
CGTCGTA3050.014.55737610
TAACGAA2550.014.50980413
CGGACCA4250.014.3647069
TACCGTC3500.014.2714287
CGCAAGA4850.014.1134022
Produced by FastQC (version 0.11.5)