FastQCFastQC Report
Thu 28 Apr 2016
ERR1042043.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042043.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2144678
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT57110.26628706034192545No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT51480.24003603338123486No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41690.19438815523822223No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22700.10584339467276672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG1200.023.12499835
TAACGGC1355.638867E-1119.18518636
TGTGCGA609.2403736E-418.510
CTAACGC650.001580537417.0769233
TCTAGCG2500.017.0228
GGACCGT1352.2222594E-816.4444456
TATACTG4050.015.9876555
ACGGACC5050.015.7524758
GGTATCA28500.015.2543861
AATCGTC1101.4529549E-515.13636428
CTAGCGG2850.014.92982629
GTATTAG7600.014.8486841
ATACCGT3750.014.7999996
CGACGGT3650.014.698637
TGACCCG3650.014.6986313
GACGGAC5200.014.5865387
AAGACGG5500.014.4636365
TACGACG3850.014.4155845
ACGACGG3750.014.3066666
CGGACCA5700.014.2807039
Produced by FastQC (version 0.11.5)