FastQCFastQC Report
Thu 28 Apr 2016
ERR1042042.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042042.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1328372
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33580.252790633948924No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29080.21891458115648327No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24980.188049733056704No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13670.10290792037170311No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11400.023.6929841
ACACCGT1005.347829E-1022.1999996
TGATCGA450.003826116420.55555710
CGTATTA609.237849E-418.515
CGAACGA1054.798658E-717.61904716
ACGAACG1107.809849E-716.81818215
TCTATAC2350.016.5319163
TCGTTTA1751.3096724E-1015.85714230
AACGCCG1751.3096724E-1015.8571425
CGCATCG1902.7284841E-1115.57894613
GCGTTAT2150.015.4883721
TAACGCC1802.0190782E-1015.4166674
GACGGAC2550.015.2352947
CGTTATT1954.1836756E-1115.1794872
TCGCCGG1853.0559022E-1015.017
ATAGACG750.004104854514.7999993
ACCGTCG1904.5474735E-1014.6052628
CGATAAC1405.996353E-714.53571410
TACCGTC2059.276846E-1114.4390257
GGTTTAG1803.3305696E-914.3888891
Produced by FastQC (version 0.11.5)