FastQCFastQC Report
Thu 28 Apr 2016
ERR1042041.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042041.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10317260
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT242890.23542103232835074No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT184880.17919486375258548No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT175770.17036500000969249No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80250.022.453581
CTAGCGG14450.016.38754529
TAACGGC3850.015.37662336
TCTAGCG15100.015.3145728
CGCAATA15300.015.11437936
TATACTG21100.015.0805695
TACACCG7650.014.99346355
GACGGAC21600.014.4745387
AAGACGG24050.014.4615375
GTATCAA124600.014.4169332
TTAACGG3850.014.41558435
TCGTTTA13350.014.41198530
GTATTAG27600.014.2771741
CAAGACG25150.014.1968184
ACGGACC22950.014.1067548
CGTTATT17400.013.6091952
GTAAACG8200.013.53658527
ATTGACG21650.013.50115532
GCGTTAT19550.013.3427111
TTGACGG21850.013.123569533
Produced by FastQC (version 0.11.5)