FastQCFastQC Report
Thu 28 Apr 2016
ERR1042040.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042040.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5264757
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT206510.392249822736358No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT177650.3374324778902426No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT165550.31444946082031894No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68980.13102219152754818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65300.022.1831
TCTAGCG6300.018.528
TATACTG11200.016.8482155
CGCAATA7100.016.67605836
CTAGCGG6900.016.62318829
ACGGACC9650.016.4870478
GACGGAC10050.016.1990057
AAGACGG10950.015.543385
TGCGACG1101.4537534E-515.13636422
CGGACCA10650.015.1126769
TATACCG1601.0990334E-815.0312515
AGACGGA10950.014.3607316
GTATCAA101400.014.2307692
TTATACT11600.014.0344834
ATACCGC9150.013.9508227
CGCAAGA11850.013.8945142
TAGCGGC8550.013.84795330
GTATAGA8350.013.73652651
CTAGTAC3000.013.5666673
TACCGCA9550.013.56020928
Produced by FastQC (version 0.11.5)